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Louis, USA) cells were cultured in DMEM/F12 growth medium (Gibco, USA) supplemented with FBS (10% v/v) and antibiotic solution (100 U/mL penicillin, 100 g/mL streptomycin) and incubated at 37 C in a humidified chamber with 5% CO2 31
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a-b , HCC-1806 cells were transduced with CRISPRi lentiviral vector containing sgRNA guides targeting the GGT1 promoter
Over time, this cumulative oxidative stress impairs mitochondrial function, disrupts cell signaling, and promotes inflammation - all hallmarks of biological aging
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